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| Mori,Enio; Lara,Maria do Carmo C.S.H.; Cunha,Elenice M.S.; Villalobos,Eliana M.C.; Mori,Claudia M.C.; Soares,Rodrigo M.; Brandão,Paulo E.; Fernandes,Wilson R.; Richtzenhain,Leonardo J.. |
| Partial nucleotide sequences of ORF72 (glycoprotein D, gD), ORF64 (infected cell protein 4, ICP4) and ORF30 (DNA polymerase) genes were compared with corresponding sequences of EHV-1 reference strains to characterize the molecular variability of Brazilian strains. Virus isolation assays were applied to 74 samples including visceral tissue, total blood, cerebrospinal fluid (CSF) and nasal swabs of specimens from a total of 64 animals. Only one CSF sample (Iso07/05 strain) was positive by virus isolation in cell culture. EHV-1 Iso07/05 neurologic strain and two abortion visceral tissues samples (Iso11/06 and Iso33/06) were PCR-positive for ORF33 (glycoprotein B, gB) gene of EHV-1. A sequence analysis of the ORF72, ORF64 and ORF30 genes from three EHV-1... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Equid herpesvirus type 1; Equid Brazilian herpesvirus; ICP4 gene (ORF64); Glycoprotein D gene (ORF72); DNA polymerase gene (ORF30). |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200565 |
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